PCR Protocols: A Guide to Methods and ApplicationsMichael A. Innis Academic Press, 1990 - 482 páginas The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to: * Optimize novel applications * Avoid many cumbersome molecular biological techniques * Set up the laboratory to avoid contamination |
Contenido
Amplification of Genomic DNA | 13 |
Amplification of RNA | 21 |
Rapid Amplification of cDNA Ends | 28 |
Derechos de autor | |
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PCR Protocols: A Guide to Methods and Applications Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White Vista previa limitada - 2012 |
PCR Protocols: A Guide to Methods and Applications Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White Sin vista previa disponible - 1990 |
Términos y frases comunes
1990 by Academic Acad Academic Press agarose gel aliquots alleles amplification products amplified DNA analysis annealing Applications Copyright assay cDNA cells chromosome cloning concentration containing cycles denaturation detection digestion DNA fragments DNA sequences dNTP dot blot EDTA electrophoresis enzymatic amplification ethanol ethidium bromide extraction Faloona form reserved gene genetic genomic genomic DNA Guide to Methods H. A. Erlich Higuchi hybridization Incubate labeled lanes Literature Cited membrane Methods and Applications MgCl2 minutes molecular molecules mRNA mutations Natl Nucleic Acids Res nucleotide oligomer oligonucleotide probes PCR amplification PCR primers PCR product PCR Protocols Perkin-Elmer Cetus phage pmol polymerase chain reaction polymorphisms primer primer pair procedure purified quences reagents recombinant region restriction enzymes reverse transcriptase rights of reproduction rRNA Saiki Scharf single-stranded Sninsky solution Southern blot specific strand synthesis Taq DNA polymerase Taq polymerase temperature template tion tissue transcription Tris-HCl pH tube virus vitro