Methods of Enzymatic AnalysisHans-UIrich Bergmeyer Elsevier, 2012 M12 2 - 1088 páginas Methods of Enzymatic Analysis focuses on the general progress in enzymology and in the special field of enzymatic analysis. This book explores the commercial production of biochemical reagents for analysis and explains the transition from the possible use of enzymatic analysis to its various applications in pure and applied biochemistry. Organized into four sections, this book starts with an overview of the basis of enzymatic analysis and provides general experimental guidelines for the techniques of measurement and for the disintegration of cells and tissues. This text then provides detailed instructions for the determination of substrates and assay of enzyme activities. Other chapters explore the practical aspects and information necessary for the application of reagents to enzymatic analysis, including sources, stability, and purity required. The final section describes the commercially available enzymes, coenzymes, substrates, and several less common reagents. Biochemists, biophysicists, researchers, and graduate students will find this book extremely useful. |
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a-oxoglutarate acetate activity in serum ammonium sulphate ammonium sulphate solution amount of enzyme assay mixture Biochem biol Bücher buffer solution Calculations catalyses centrifuge Chem Chemistry chloride clin CoA-SH commercial preparation concentration containing control cuvette crystalline cuvette deproteinized determination dilute Dissolve doubly distilled water E₁ EDTA enzyme enzyme activity enzyme preparation enzyme solution ethanol experimental cuvette extract final volume GIDH glucose glucose-6-phosphate hexokinase hydrolysis hydroxide incubation Klin lactic dehydrogenase light path liver method NaOH nucleotide obtained optical density optical density E2 oxidation perchloric acid phosphatase phosphate buffer Pipette Potassium Potassium dihydrogen phosphate precipitate Preparation of Solutions protein protein/ml pyruvate read the optical Reagents Reduced diphosphopyridine nucleotide room temperature sample Sodium hydroxide sodium salt solution VII Spectrophotometric Spectrophotometric measurements Stability standard curve standard solution substrate supernatant suspension tissue TPNH trichloroacetic acid triethanolamine tris buffer trypsin units/ml Wavelength Wróblewski Wschr ΔΕ µmoles